Biotechnology - A Laboratory Course.pdf

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Biotechnology
A Laboratory Course
Second Edition
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Biotechnology
A Laboratory Course
Second Edition
Jeffrey M. Becker
Department of Microbiology
Department of Biochemistry, Cellular,
and Molecular Biology
The University of Tennessee
Knoxville, Tennessee
Guy A. Caldwell
Department of Biological Sciences
Columbia University
New York, New York
Eve Ann Zachgo
Department of Molecular Biology
Massachusetts General Hospital
Boston, Massachusetts
Academic Press
San Diego New York
Boston London
Sydney Tokyo
Toronto
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Front cover photograph: Bacteria and yeast, the work horses ofbiotechnology and molecular
biology, are depicted as a bacterial colony and one budding yeast cell containing plasmid DNA
and the DNA double helix. This illustration represents the manipulation of living organisms to
produce material useful for humans - a definition ofbiotechnology in the broadest sense. The
recent revolution in this field of endeavor has been made possible by the explosion of
knowledge in molecular biology and by the rapid proliferation of techniques using
microorganisms and recombinant DNA.
This book is printed on acid-free paper. (~
Copyright 9 1996, 1990 by ACADEMIC PRESS
All Rights Reserved.
No part of this publication may be reproduced or transmitted in any form or by any
means, electronic or mechanical, including photocopy, recording, or any information
storage and retrieval system, without permission in writing from the publisher.
Permissions may be sought directly from Elsevier's Science & Technology Rights
Department in Oxford, UK: phone: (+44) 1865 843830, fax: (+44) 1865 853333,
e-mail: permissions@elsevier.com.uk. You may also complete your request on-line
via the Elsevier Science homepage (http://elsevier.com), by selecting "Customer
Support" and then "Obtaining Permissions."
Academic Press
An imprint of Elsevier Science
525 B Street, Suite 1900, San Diego, California 92101-4495, USA
http://www.academicpress.com
Academic Press
84 Theobald's Road, London WC1X 8RR, UK
http://www.academicpress.com
Library of Congress Catalog Card Number: 95-41427
International Standard Book Number: 0-12-084562-8
PRINTED IN THE UNITED STATES OF AMERICA
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